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Objective@#This study was performed to compare the genetic diversity, virulence, and antimicrobial resistance of @*Methods@#A total of 38 clinical strains and 19 strains from healthy individuals were isolated from the samples collected in Ma'anshan City, Anhui Province. Their taxonomy was investigated using concatenated @*Results@#The 57 @*Conclusions@#The taxonomy, virulence properties, and antibiotic resistance of
Subject(s)
Humans , Aeromonas/pathogenicity , Case-Control Studies , Drug Resistance, Bacterial/genetics , Genetic Variation , Virulence Factors/geneticsABSTRACT
Objective: To analyze the registration characteristics of registered clinical research, and find the potential scientific and feasibility problems of clinical research on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), providing reference for the good management of follow-up research registration. Methods: The key words of coronavirus disease 2019 (COVID-19) were identified and retrieved from Chinese Clinical Trial Registry and ClinicalTrials website. The registration characteristics of ethical status, number of recruits, total time, number of groups, intervention, study endpoint type, withdrawal, randomized controlled trial (RCT), stage, registration type, provinces distribution and patients' condition were summarized. IBM SPSS 22.0 software was used to analyze the above characteristics. Results: A total of 400 registered clinical studies were collected. Among them, 59 studies were not ethically approved, 15 studies were withdrawn, and stages of 303 studies were unclear. The differences of the three registration characteristics on the two official websites were statistically significant (all P<0.05). Fourteen studies recruited more than 1 000 people, the total time of 189 studies exceeded 6 months, and the number of groups in 22 studies exceeded 4 groups. There was no significant difference in the three registration characteristics on the two official websites. Only 15 studies were industry-sponsored trial. Most registered clinical studies were distributed in Hubei Province. Conclusion: The awareness of Chinese investigator initiating trial registration has increased. However, by collating and analyzing the registration information, it is found that the study design is not rigorous, so it is necessary to strengthen the registration quality management and study design methodological demonstration.
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Objective: To explore the effects of eriCF1 and eriCF2 genes under the condition of different fluoride concentrations on the fluoride resistance of Streptococcus mutans (S. mutans) fluoride-resistant strain UA159-FR, and to elucidate the relationship between the differential expressions of eriCF genes and the fluoride resistance of S. mutans fluoride-resistant strain. Methods: The recombinant E. coli competent cells BL21 were divided into eriCF1 group (eriCF1+ Peasy-Blunt E2), eriCF2 group (eriCF2+ Peasy-Blunt E2) and KZ group (Peasy-Blunt E2). After gene cloning and IPTG induction, the expression levels of EriCF1 and EriCF2 proteins in three groups were detected, and the concentrations of bacterial solution and the growth rates of bacterium of strains in three groups were determined under different concentrations of fluoride conditions (1. 5, 2.0, 2.5, and 3. 0 g middot; L-1 sodium fluoride); the growth curves were drawn. Results: At logarithmic phase (8 h) and stable phase (16 h), the final concentrations of bacterial solution and the growth rates of the strains in three groups under different concentrations of fluorine environments (1. 5, 2. 0, 2. 5 and 3. 0 g middot; L-1 sodium fluoride) were compared in the same group, and the final concentrations and the growth rates of bacterial solution of the strains in three groups were decreased with the increasing of sodium fluoride concentration; the final concentrations and the growth rates of bacterial solution results were eriCF2 group > eriCF1 group > KZ group (P<0. 05 or P<0. 01).compared between three groups under the same concentration of sodium fluoride, the concentration of bacterial solution in eriCF2 group was increased significantly compared with eriCF1 group (P<0. 01). Conclusion: Both eriCF1 and eriCF2 genes can enhance the fluoride resistance of E. coli, and the fluoride resistance of E. coli expressing eriCF2 gene is stronger than that of E. coli expressing eriCF1 gene.
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<p><b>OBJECTIVE</b>To study the regulatory effect of Bushenfang on the serum testosterone (T) level of naturally aging rats and its mechanism, in order to provide a theoretical and experimental basis for the clinical treatment of late onset hypogonadism (LOH) in males.</p><p><b>METHODS</b>Thirty-two 18-month-old male SD rats were randomly divided into four groups of equal number, naturally aging model and low-, medium- and high-dose Bushenfang groups, and another eight 4-month-old rats were taken as normal controls. The rats of the aging model and normal control groups were treated with normal saline, while those of the low-, medium- and high-dose Bushenfang groups received intragastrically Bushenfang at 3.25, 7.50 and 15.00 g/kg, respectively, all for 3 weeks. Then the rats were sacrificed, the histomorphologic changes of the testis observed by HE staining, the serum T level measured by radioimmunoassay, and the expressions of the StAR protein, P450scc and 3beta-HSD I determined by RT-PCR.</p><p><b>RESULTS</b>The number of Leydig cells was obviously increased after Bushenfang treatment. The levels of serum T were significantly higher in the low-, medium- and high-dose Bushenfang groups ([6.74 +/- 1.56] nmol/L, [8.50 +/- 1.99] nmol/L and [12.41 +/- 2.91] nmol/L) than in the model group ([3.48 +/- 0.75] nmol/L) (P < 0.05). The three Bushenfang groups also showed a remarkable elevation in the mRNA expressions of StAR (0.74 +/- 0.29, 0.83 +/- 0.32 and 1.35 +/- 0.50), P450scc (0.72 +/- 0.36, 1.023 +/- 0.30 and 1.41 +/- 0.37) and 3beta-HSD I (0.58 +/- 0.14, 0.72 +/- 0.07 and 0.85 +/- 0.18), as compared with the models (StAR: 0.44 +/- 0.09; P450scc: 0.33 +/- 0.05; 3beta-HSD I: 0.34 +/- 0.02), with significant differences in the StAR expression between the high-dose Bushenfang and the model groups, as well as in P450scc and 3beta-HSD I expressions between the medium- and high-dose Bushenfang and the model groups (P < 0.05).</p><p><b>CONCLUSION</b>Bushenfang could improve the pathological status of testicular injury and increase the expression of testosterone synthetase, which might be the mechanism behind its regulatory effect on the serum T level of aging rats.</p>